Cellular Quality Control Mechanism: A Survival company under multifactorial toxic events: Protein ubiquitylation is highly versatile, ordered and multistep post-translational modification enzymatic process that regulates numerous aspects of cell physiology. Tight control of numerous cellular pathways is a vast and responsible task. Failure of a single component of cellular quality system disturbs whole network. This trouble further leads human pathogenesis, including neurodegeneration, tumor initiation, and progression.To facilitate our research, we are combining various latest biochemical technique’s that enable us to explore functional aspects of E3 Ubiquitin Ligases biology in a comprehensive way. Current efforts strive for identification, assessment, and characterization of E3 Ubiquitin Ligases substrates linked with the pathogenesis of neurodegenerative diseases, neurodevelopmental disorders, and cancer. The overall goal of our research is to understand how confidential network of various E3 Ubiquitin Ligases integrate quality control mechanism in normal cells, and how mutations that disrupt these processes impact disease development.
Investigating the cellular signaling of ubiquitin ligases implicated in cell cycle regulation and cancer pathways: The ubiquitin-proteasome system (UPS) regulates several cellular functions (Hershko and Ciechanover, 1998). In UPS ubiquitin E3 ligases acquire a very important function they directly bind and recognize specific cellular substrates (Petroski and Deshaies, 2005). Ubiquitin ligase mediated growth inhibition and senescence is widely accepted as a cellular defensive response against tumorigenesis (Nakayama and Nakayama, 2006). Acknowledging the efficiency of the cellular quality control system, it is expected that there might be an existence of early quality control system in cells which is governed by E3 ubiquitin ligases. However, the precise role of E3 ubiquitin ligases in cancer pathobiology is not clear. Inhibitors and activators of these ubiquitin ligases are clinically significant as a tumor suppressor agent. This project will surely help to better assimilate the oncogenic potential of E3S. Current research will sure facilitate identification and development of individual E3S-diagnositic marker and anticancer drug targets.